OBSERVATIONS ON THE CYTOPLASMIC AND VACUOLAR ORTHO-PHOSPHATE POOLS IN LEAF TISSUES USING INVIVO P-31-NMR SPECTROSCOPY

摘要

A full understanding of the central role of Pi in photosynthesis requires information on the size of the endogenous Pi pools and the extent to which they interact with each other under different physiological conditions. In vivo 31P-NMR spectroscopy has the potential to assist in this objective but the early applications of this technique to leaf tissues were disappointing. Here we report 31P-NMR data from maize and tomato leaf discs that: (i) cast doubt on earlier estimates of the cytoplasmic orthophosphate pool size; and (ii) prove that exogenously supplied D-mannose can reduce the cytoplasmic Pi level in some circumstances. © 1989.